Monoclonal mouse anti-human insulin

D4B8cc

Mouse monoclonal antibody produced in bioreactor.  Hybridoma clone derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice.

C7C9,  7F8

Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen

cells of Balb/c mice immunized with purified human insulin.

Insulin, human

MAb C7C9 reacts with C-terminal pentapeptide of insulin β-chain. MAbs 7F8,  andD4B8cc reacts with different antigenic determinants.

These antibodies cross-react with human proinsulin, bovine insulin (30%) and porcine insulin. No

cross-reaction with free C-peptide.

IgG1, kappa for MAbs C7C9, 7F8, D4B8cc

Detection of insulin in two-site enzyme immunoassay. Following pairs are recommended

(capturedetection): 7F8 – D4B8cc

MAb D4B8cc can be used in immunohistochemistry on frozen sections.

Chromatography on protein A Sepharose

PBS, pH 7.4, 0.1 % sodium azide (NaN3)

+ 4 °C

This product is sold as an antibody preparation for research use only. Standard Laboratory

Practices should be followed when handling this material.

Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small

appropriate care must be taken when handling this product.

Rat C-peptide and proinsulin Assay Notes